P.A.S Periodic Acid Schiff Staining Hotchkiss Mc Manus

This is a simple staining using PAS stain kit. READ MORE HERE

  1. Bring section to distilled water.
  2. Put on the section 10 drops of reagent A: leave to act 10 minutes.
  3. Wash in distilled water.
  4. Put on the section 10 drops of reagent B: Leave to act 20 minutes.
  5. Wash in distilled water.
  6. Put on the section 10 drops of reagent C: Leave to act 2 minutes.
  7. Drain the slides without washing add 10 drops of reagent D: Leave to act for 2 minutes.
  8. Rinse in distilled water.
  9. Put on the section 10 drops of reagent E: 3 minutes.
  10. Wash in running tap water for 5 minutes.
  11. Dehydrate through ascending alcohols. Clear in xylene and mount.
Results
Positive PAS substance – Magenta red
Nuclei – Blue

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