How to do simple Gram Staining

Hi all,

Gram staining is where a microorganisms is coloure READ MORE HERE. I refuse to talk into the details as much can be found on the net. So here are the basics of Gram Staining

Preparation – put 2 drops of normal saline onto a glass slides. Put a small, teeny, tiny cultures onto the drops of saline. Mix the microbes with the saline. Heat Fix the slides. The slides are now ready for staining.

Gram Staining –

  1. Flood using Crystal Violet for 30sec and wash with running water
  2. Flood using Grams Iodine for 30sec and wash with running water
  3. Flood using Grams Iodine Acetone for 3sec and wash with running water. Watch out for this step as more than 3sec would render all your stain worthless.
  4. Flood using Gram Safranin for 30sec and wash with running water
  5. Blot dry using blotting paper and ready to be viewed under microscope.

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